In the present study , blood samples of 90 unrelated han chinese individuals from shandong province and 90 mongols from inner mongolia were collected randomly and genomic dna was extracted 本研究随机采取山东汉族和内蒙古蒙古族两个人群共180个个体的血液样本,提取基因组dna 。
The genomic dna of ginkgo extracted by ctab method is light brown in color . it is neither digested with restriction endonucleases nor acted as template for polymerase chain reaction ( pcr ) 常规的ctab法提取的银杏基因组dna呈黄色,不易被内切酶所消化,也不能作为pcr模板。
With the improved method of ctab , the genomic dna extracted form plum and other species close to p . mume in genetic relationship could be directly used in pcr amplification , with good results obtained 选用改良ctab法提取的梅及其近缘种的基因组dna可直接用于pcr扩增分析,且效果良好。
And strain ss - ori is sinorhizobium sp . according to the " blast " data . in addition , a hydantoinase gene ( 1440bp ) from yz - ii6 was amplified by pcr with genomic dna as the template 我们还利用pcr方法从菌株yz - 6基因组中克隆得到了海因酶的基因( 1440bp ) ,并构建了表达质粒pet3a - hdtase 。
Therefore , research on cholesterol oxidase has become more and more important . a 1 . 6 kb dna fragment was amplified using pcr method from genomic dna of rhodococcus equi . 4 - 2g2 本研究采用pcr方法从分泌胆固醇氧化酶的马红球菌菌株4 ? 2g2基因组dna中,扩增出特异dna片段,该扩增片段全长约1 . 6kb 。
Used the genomic dna extracted by low melting - point agarose embedding method as pcr template , the full length of structural genes of bacillus subtilis bio operon were gained by long pcr method 将该方法提取的基因组dna稀释100倍作为模板,采用长距离pcr方法,获得了枯草杆菌生物素操纵子基因全长。
In the experiment pea genomic dna were intrauced into wheat lu22 through the pollen tube pathway in order to enhance wheat protein content and improve wheat quality 本研究通过花粉管通道法将豌豆基因组dna导入高产低蛋白小麦品种鲁麦22号,以期提高其蛋白质含量,并探讨该方法在改良小麦品质方面的作用。
Thl1 gene was also amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 ) 采用pcr和rt - pcr技术,从甘蓝和油菜基因组dna和柱头总rna中扩增获得了719bp的thl1基因和430bp的cdna序列。
Asqs is 70 % , 77 % , 44 % and 39 % identical to squalene synthases from arabidopsis thaliana , tobacco , human and yeast , respectively . the asqs genomic dna has a complex organization containing 14 exons and 13 introns 青蒿鲨烯合酶氨基酸序列与拟南芥、烟草、人类、酵母鲨烯合酶的一致性分别为70 、 77 、 44 、 39 。
Two oligonucleotides were synthesized according to the sequence of p . furiosus extracellular a - amylase gene amya through the genbank and used as primers for pcr with p . juriosus genomic dna as the template 根据genbank公布的p furiosus的-淀粉酶基因amya序列设计两条引物,以p furiosus的基因组dna为模板进行pcr 。